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| Place of Origin: | China |
| Brand Name: | Leji |
| Model Number: | ljsw001 |
| Minimum Order Quantity: | Negotiation |
|---|---|
| Price: | Negotiation |
| Packaging Details: | Insulation with ice packs to ensure stable enzyme activity |
| Delivery Time: | 7-15 working days |
| Payment Terms: | T/T, western union, moneygram |
| Supply Ability: | 5 - 25 metric ton per month |
| CAS No.: | 72561-05-8 | Other Names: | Endoproteinase Lys-C |
|---|---|---|---|
| MF: | C30H27N3O5 | EINECS No.: | 3.4.21.50 |
| Type: | Vitamins, Amino Acids And Coenzymes, Auxiliaries And Other Medicinal Chemicals | Grade Standard: | Cosmetic Grade, Medicine Grade |
| Port: | ShangHai Port | ||
| High Light: | argireline powder,pharmaceutical formulation intermediates |
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Silktightin Peptide Powder Stock Solution Cosmetic Material Anti Wrinkle
Recombinant Lysyl Endopeptidase
Product Description
Lysyl Endopeptidase (Lys-C,EC 3.4.21.50) belongs to a serine protease that specifically cleaves peptide bonds at the carboxy terminus of lysine residues. Lysyl Endopeptidase is widely used in biopharmaceutical, biological analysis, cell culture and other fields.
This product is a lyophilized powder expressed by recombinant E. coli, freeze-dried by multi-step separation and purification, and has the same activity and specificity as natural Lysyl Endopeptidase. This product is a biochemical grade genetically engineered enzyme which does not contain enzyme inhibitors such as DFP, PMSF and TLCK. Serine protease inhibitors such as PMSF inhibit enzyme activity, but metal ion chelators such as EDTA do not inhibit enzyme activity.
Product features
l Easy to detect residual amount:It can be used to detect residues in commercial kits, especially in the pharmaceutical industry.
l No animal origin:The product is free from virus contamination and does not use any animal derived materials during the production process.
l High activity and low dosage:≥3.0AU/mg pro.
l High substrate specificity: only recognizes and cleaves lysine residues.
l Strong tolerance:wide range of pH tolerance and strong surfactant tolerance.
l Lyophilized powder: easy to transport and store.
l Consistent quality:It can guarantee continuous and stable mass production, and the quality difference between batches is small.
l Compliance:Production equipment and production environment must comply with relevant regulations and comply with GMP guidelines.
l Complete quality documentation:Relevant regulatory support documents are available upon request.
Product Characteristics
|
Source |
Escherichia coli |
|
Appearance |
White or off-white powder |
|
Molecular weight |
29KD±2.9KD |
|
Purity |
Single main band (SDS-PAGE). |
|
Exogenous DNA residue |
Less than 100ng/mg protein |
|
HCP residue |
Less than 100ng/mg protein |
|
Specific activity |
≥3.0AU /mg pro. |
Unit Definition 30 ° C, pH 9.5, the enzyme amount of 1 μmol of p-nitroaniline per minute of catalytic substrate is 1 AU.
| Product Characteristics |
l Used for the production of biological products, such as insulin and analog production, GLP-1 analogue production.
l Used for protein structure and function studies, such as protein mass spectrometry, sequencing, peptide mapping analysis.
l Used for production of small molecular proteins or peptides, such as cosmetic peptides, antimicrobial peptides, food flavor peptide production.
l Used for enzymatic synthesis for Lys-X.
l Used for cultivation of stem cells and primary cells, with milder effecttion.
Usage method
l It is recommended to dissolve the enzyme using 20mM Tris of pH8-10. Dissolve the protein or peptide in buffer,and adjust the pH to the optimum pH 9.0-10.0,then add Lys-C with ratio of 2~100AU/g peptide or protein and incubate sample for 2~18 hours at 25~ 35°C.
l Precautions:Inorganic salts above 0.1M have a certain effect on the enzyme activity, and it is recommended to carry out the enzyme digestion reaction after desalting. If it is not possible to desalinate, it is recommended to increase the amount of enzyme and extend the digestion time.
Transportation and storage stability
l Transport stability:Insulation with ice packs to ensure stable enzyme activity.
l Storage stability: Stored at -15 ° C to -20 °C and can be stable for 24 months. When Tris-HCl buffer is formulated into an enzyme solution, it can be stably stored for at least 24 hours at 4 ° C.
Related products
Recombinant Lysyl Endopeptidase Lyophilized powder (with His tag);Recombinant Lysyl Endopeptidase Lyophilized powder;Recombinant Lysyl Endopeptidase Lyophilized powder (Sequencing Arade);Recombinant Trypsin Lyophilized powder;Recombinant Carboxypeptidase B.
product specifications
|
Item number |
Specification |
|
P302-10AU |
10AU/Vial |
|
P302-100AU |
100AU/Vial |
|
P302-1000AU |
1000AU/Vial |
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